Differential Display Method Using Chemi - luminescent Detection
نویسنده
چکیده
the LCLINK-D assembly. The sluggishness of the VHICH1-LINK-D reaction may be caused by formation of strong secondary structures within the coding region of CHI, which would also account for the relatively weaker amplification of the Fd gene (as compared to the VH gene) as observed previously (9). Although the PCR assembly strategy has been described as somewhat difficult (4), we find it an attractive alternative to the cloning of heavyand light-chain genes separately. Furthermore, the rare cutting-restriction enzymes S ' I and Not1 are the only enzymes used in the entire cloning procedure. Use of more enzymes inevitably increases the danger of cleavage within the Fab coding regions, which would lead to less comprehensive libraries.
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